IRTG seminar "Protein expression and purification"


2024-10-09    09:00 - 12:00

IRTG students

Seminar by Dr. Sabine Schneider

Topics covered:

- protein sequence, isoforms (Uniprot, Blast, HHPRED, ...)

- structure prediction, flexible/disordered regions, signal peptides, cofactors, metal ions etc.)

- expression systems (E. coli (mainly), insect cells (briefly))

- test expression (media, temperature, E. coli strains, ...)

- purification: affinity purification (His-tag, Strep-tag), ion-exchange and size exclusion chromatography

- proteases for tag-removal (TEV, Precission, Sumo, thrombin)

- choice of buffer

- quality control (SDS-PAGE, DLS, Thermofluor, MS, ...)

- trouble shooting

Campus Großhadern, ICEM, Würmtalstr. 201, 81377 München