IRTG students
Seminar by Dr. Sabine Schneider
Topics covered:
- protein sequence, isoforms (Uniprot, Blast, HHPRED, ...)
- structure prediction, flexible/disordered regions, signal peptides, cofactors, metal ions etc.)
- expression systems (E. coli (mainly), insect cells (briefly))
- test expression (media, temperature, E. coli strains, ...)
- purification: affinity purification (His-tag, Strep-tag), ion-exchange and size exclusion chromatography
- proteases for tag-removal (TEV, Precission, Sumo, thrombin)
- choice of buffer
- quality control (SDS-PAGE, DLS, Thermofluor, MS, ...)
- trouble shooting
Campus Großhadern, ICEM, Würmtalstr. 201, 81377 München
Speaker: Prof. Dr. Thomas Carell
Ludwig-Maximilians-Universität München
Institut für Chemische Epigenetik (ICEM)
Department of Chemistry
Office:
Würmtalstrasse 201
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77750
Fax: +49 (0)89 2180-77756
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Management: Dr. Nada Raddaoui
Institute for Chemical Epigenetics Munich (ICEM)
Office:
Würmtalstrasse 201, Building L, Room 03.004
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77755
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Secretary: Birgit Carell
Institute for Chemical Epigenetics Munich (ICEM)
Office:
Würmtalstrasse 201, Building L, Room 00.007
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77751
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
website support: Martina Schuster
Prof. Dr. Lena Daumann
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House D, Room 3.075
81377 Munich, Germany
Phone: +49 89 2180 77486
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Dr. Sabine Schneider
LMU Munich
Institute for Chemical Epigenetics
Butenandtstr. 5 - 13
House L
81377 Munich, Germany
Phone: +49 89 2180 77716
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Dr. Martin Sumser (Coordinator)
LMU Munich
Institute for Chemical Epigenetics
Butenandtstr. 5 - 13
House L
81377 Munich, Germany
Phone: +49 89 2180 77765
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Contact IRTG 1309 related
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