A set of rhamnosylation-specific antibodies enables detection of novel protein glycosylations in bacteria


Daniel Gast, Franziska Koller, Ralph Krafczyk, Lukas Bauer, Swetlana Wunder, Jürgen Lassak and Anja Hoffmann-Röder

Org. Biomol. Chem., 2020, 18, 6823–6828


Despite its potential importance for bacterial virulence, protein rhamnosylation has not yet been sufficiently studied. Specific anti-SerRha, anti-ThrRha and anti-AsnRha antibodies allowed the identification of previously unknown monorhamnosylated proteins in cytosol and membrane fractions of bacterial cell lysates. Mapping of the complete rhamnoproteome in pathogens should facilitate development of targeted therapies against bacterial infections.