2024-08-15

Sandra Nitsch & Robert Schneider

Part of the book series: Methods Mol Biol 2846:1-16

https://doi.org/10.1007/978-1-0716-4071-5_1

For the genome-wide mapping of histone modifications, chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing remains the benchmark method. While crosslinked ChIP can be used for all kinds of targets, native ChIP is predominantly used for strong and direct DNA interactors like histones and their modifications. Here we describe a native ChIP protocol that can be used for cells and tissue material.