Area A: Modifications on Nucleic Acids

Project A03 – The effect of DNA modifications on the stability and mobility of nucleosomes

In addition to the canonical DNA bases chemically modified bases have been found recently. In particular for 5-fC a role in epigenetics is certain. How this modification affects gene expression is so-far elusive. On one hand, we will characterize potential changes in structure and dynamics of DNA double strands upon inclusion of 5-fC. On the other, changes in DNA-histone interactions in nucleosomes due to 5-fC will be determined. For these goals, we use NMR spectroscopy in solution and in the solid state.