Area B: Modifications on Proteins

Project B01 – O-GlcNAc profiling and epigenetic regulation

The dynamic O-GlcNAcylation at specific protein sites is controlled by the two enzymes Ogt and Oga, with the former closely interacting with Tet proteins during epigenetic regulation. Since very little is known about this O-GlcNAc-based Ogt-Tet cross-talk, we plan to map O-GlcNAcylated proteins at specific cellular differentiation stages using chemical labeling and modern MS-based GlcNAcomic techniques. Thus, by developing novel metabolic labels and analyzing specific O-GlcNAcylation profiles of Tet proteins and their binding partners, we hope to decipher the molecular basics of these regulative Ogt-Tet interactions.