SFB1309 Lecture in collaboration with CNATM and CAS: Prof. Dr. Cynthia Burrows

2024-04-10    17:15

Prof. Dr. Cynthia Burrows is Thatcher Presidential Chair and Distinguished Professor
Department of Chemistry, University of Utah, Salt Lake City

Title: "Nanopore Studies on Chemically Modified RNA from Viruses to Vaccines"

Using nanopore technology we have been able to apply direct RNA sequencing for chemical modifications by inspecting the data for base-calling errors, ionic current and dwell-time differences to map up to 17 modifications in 35 locations of the E. coli ribosome. For pseudouridine, these methods can be enhanced by modification-specific chemistry based on bisulfite. Application of these methods to viral RNA led us to identify conserved sites of pseudouridinylation of SARS-CoV-2 genomic RNA, and we could further sequence RNA synthesized by IVT in which m1Ψ replaces some of the U nucleotides with a view toward more effective mRNA vaccines. These results are a major step toward the Holy Grail of single-molecule sequencing for simultaneous identification and quantification of all nucleotide chemical modifications in RNA.

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