Project B03 – Dynamics and function of posttranslational protein methylation and acetylation
The methylation and acetylation of lysine residues within proteins that regulate gene expression has been shown to play a key role in various diseases and the enzymes that catalyze these modifications have therefore gained considerable interest as potential drug targets. Surprisingly little is known about the proteins that are modified, the regulation of the enzymes, the dynamics of their turnover or their functional implications. Within this project we plan to use a variety of biochemical strategies to determine the complete lysine methylome of a human cell line. We will furthermore investigate the substrate specificity of a set of putative methyltransferases by applying a combination of different in vitro and in vivo assays. In addition, we will establish MALDI imaging as a new technology to investigate the distribution of inhibitors targeting such modifying enzymes and simultaneously the corresponding histone proteoform within tissues. Finally, we aim to use MALDI imaging as a new and antibody independent way to detect, quantify and characterize modified nucleic acids in situ.
Speaker: Prof. Dr. Thomas Carell
Ludwig-Maximilians-Universität München
Institut für Chemische Epigenetik (ICEM)
Department of Chemistry
Office:
Würmtalstrasse 201
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77750
Fax: +49 (0)89 2180-77756
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Management: Dr. Nada Raddaoui
Institute for Chemical Epigenetics Munich (ICEM)
Office:
Würmtalstrasse 201, Building L, Room 03.004
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77755
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Secretary: Birgit Carell
Institute for Chemical Epigenetics Munich (ICEM)
Office:
Würmtalstrasse 201, Building L, Room 00.007
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77751
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website support: Martina Schuster
Prof. Dr. Lena Daumann
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House D, Room 3.075
81377 Munich, Germany
Phone: +49 89 2180 77486
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Dr. Sabine Schneider
LMU Munich
Institute for Chemical Epigenetics
Butenandtstr. 5 - 13
House L
81377 Munich, Germany
Phone: +49 89 2180 77716
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Dr. Martin Sumser (Coordinator)
LMU Munich
Institute for Chemical Epigenetics
Butenandtstr. 5 - 13
House L
81377 Munich, Germany
Phone: +49 89 2180 77765
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