Area B: Modifications on Proteins

Project B05 – Role of TET3-mediated 5mC oxidation for neuronal differentiation and plasticity

The physiological significance of TET3 proteins and their enzymatic products in the CNS has not been characterized and will be addressed with specific genetic mouse and cellular models in Aim A. TET enzymes act in concert with chromatin remodeling proteins and transcription factors. In Aim B we will assess the potential of interacting proteins to engage with TET3 and modulate its enzymatic activity. The genomic 5hmC and 5fC content in neurons varies depending on differentiation and/or activity state. In Aim C we will explore the underlying mechanism of this phenomenon.