Area B: Modifications on Proteins

Project B08 – Characterisation of microtubule glycylation during neuronal differentiation

The so called “tubulin code” has emerged to be the key for organisation of a neural cytoskeleton and during development of nervous tissues. In the proposed project, we focus on microtubules glycylation, which is critical for cilia function and neuronal development. Only a little is known about the scope and function of glycylation and polyglycylation on microtubules and other non-tubulin proteins in neurons. We propose to develop a set of glycine probes for mass spectrometry-based chemical proteomics to uncover the scope and selectivity of (poly-)glycylation during neural differentiation and maturation. Our findings will contribute to better understanding of neurodevelopment and associated ciliopathies.