Area C: Mechanisms and Tools

Project C05 – Spectroscopic and mechanistic studies of Fe/α-ketoglutarate dependent oxidases for the elucidation of demethylation mechanisms

It is the aim of this subproject to examine the key steps in the catalytic cycle of AlkBH- and TET-enzymes and to compare the local perturbations in the active site caused by the binding of different substrates with a variety of spectroscopic methods. The studies will go hand in hand with the investigation of model complexes that mimic the function of TET-enzymes. It is hoped that these studies will help to unravel the structure-reactivity relationships in TET/AlkBH-enzymes relevant in epigenetic transformations.