Area A: Modifications on Nucleic Acids

Project A05 – Sequence specific and functional analysis of oxdative modifications in nucleic acids – implication for epigenetic memory in the mouse

Tet mediated oxidative modifications of 5-methylcytosine (5mC) regulate and modulate short term and long term effect of the 5mC mediated epigenetic memory formation. In this project we will analyse the formation and regulation of 5fC and 5hmC in the mouse zygote, ES cells and primary neurons of newborn and adult mice. We will develop novel methods for base specific detection of 5fC and 5hmC and will use these methods to determine the functional consequences of a loss of distinct Tet3 isoforms using NGS data based modeling.