Area B: Modifications on Proteins

Project B04 – PARP1-mediated recruitment of nucleic acid regulators and their function in DNA demethylation and the circadian clock

DNA damage recruits nuclear factors to chromatin, often in a PARP1-dependent manner. This includes Cryptochrome 1 (CRY1), a circadian clock component, METTL3/14, the m6A methyltransferase METTL3, and ATP-dependent chromatin remodelers. Moreover, PARP1 and the PAR-dependent remodeler ALC1/CHD1L promote cell reprogramming by Yamanaka factors and DNA demethylation. We will establish the mechanism(-s) through which PARylation promotes recruitment and test the role of PARylation in DNA demethylation and circadian gene expression.