Area A: Modifications on Nucleic Acids

The effect of DNA modifications on the stability and mobility of nucleosomes

In addition to the canonical DNA bases chemically modified bases have been found recently. In particular for 5-fC a role in epigenetics is certain. How this modification affects gene expression is so-far elusive. On one hand, we will characterize potential changes in structure and dynamics of DNA double strands upon inclusion of 5-fC. On the other, changes in DNA-histone interactions in nucleosomes due to 5-fC will be determined. For these goals, we use NMR spectroscopy in solution and in the solid state.