SFB 1309
The pH-Dependence of the Hydration of 5-Formylcytosine - an Experimental and Theoretical Study
2022-01-27
Hendrik Zipse, Fabian L. Zott, Vasily Korotenko
Chem. Bio. Chem., 2022, e202100651
5-Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has been implicated in the formation of 5-carboxycytosine as well as oligonucleotide binding events. The hydrate content of 5-formylcytosine and its uracil derivative has now been quantified using a combination of NMR and mass spectroscopic measurements as well as theoretical studies. Small amounts of hydrate can be identified for the protonated form of 5-formylcytosine and for neutral 5-formyluracil. For neutral 5-formylcytosine, however, direct detection of the hydrate was not possible due to its very low abundance. This is in full agreement with theoretical estimates.
