A Click‐Chemistry‐Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry
2019-10-08
Dr. Michael Stadlmeier, Leander Simon Runtsch, Filipp Streshnev, Prof. Dr. Martin Wühr, Prof. Dr. Thomas Carell
Chem Bio Chem., 2019, 21 (1-2), 103–107
Mass spectrometry is the method of choice for the characterisation of proteomes. Most proteins operate in protein complexes, in which their close association modulates their function. However, with standard MS analysis, information on protein–protein interactions is lost and no structural information is retained. To gain structural and interactome data, new crosslinking reagents are needed that freeze inter‐ and intramolecular interactions. Herein, the development of a new reagent, which has several features that enable highly sensitive crosslinking MS, is reported. The reagent enables enrichment of crosslinked peptides from the majority of background peptides to facilitate efficient detection of low‐abundant crosslinked peptides. Due to the special cleavable properties, the reagent can be used for MS2 and potentially for MS3 experiments. Thus, the new crosslinking reagent, in combination with high‐end MS, should enable sensitive analysis of interactomes, which will help researchers to obtain important insights into cellular states in health and diseases.
Speaker: Prof. Dr. Thomas Carell
Ludwig-Maximilians-Universität München
Institut für Chemische Epigenetik (ICEM)
Department of Chemistry
Office:
Würmtalstrasse 201
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77750
Fax: +49 (0)89 2180-77756
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Managing Director: Dr. Oliver Baron
Institute for Chemical Epigenetics Munich (ICEM)
Ludwig-Maximilians-Universität München
Butenandtstr. 5 - 13
81377 Munich Germany
Phone: +49 (0)89 2180-77661
Fax: +49 (0)89 2180-77651
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Secretary: Birgit Carell
Institute for Chemical Epigenetics Munich (ICEM)
Office:
Würmtalstrasse 201, Haus L - EG / Room: 007
81377 Munich
Germany
Mailing address:
Butenandtstr. 5 - 13
81377 Munich
Germany
Phone: +49 (0)89 2180-77751
Fax: +49 (0)89 2180-77881
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Prof. Dr. Hendrik Zipse (Spokesman)
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House F, Room 3.084
81377 Munich, Germany
Phone: +49 89 2180 77737
Fax: +49 89 2180-77738
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Dr. Stefanie Kellner (Deputy Spokeswoman)
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House F, Room 1.054 und House B, Room 2.073
81377 Munich Germany
Phone: +49 89 2180 77737
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Dr. Martin Sumser (Coordinator)
LMU Munich
Department of Chemistry
Butenandtstr. 5 - 13
House F, Room 4.086
81377 Munich Germany
Phone: +49 89 2180 77765
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